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CAS No.: 188532-26-5

NL-1 Catalog No. CSN25313

NL-1 is a mitoNEET inhibitor with antileukemic effect. NL-1 inhibits REH and REH/Ara-C cells growth with IC50s of 47.35 µM and 56.26 µM, respectively.

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靶点选择性

生物活性

实验方案

技术信息

生物活性

描述MitoNEET (CISD1) is a mitochondrial outer membrane protein that plays a critical role in maintaining mitochondrial homeostasis and is frequently overexpressed in cancer. NL-1, derived from pioglitazone, is a mitoNEET inhibitor with antileukemic activity. Treatment with NL-1 reduced the number of viable cells in both REH and REH/Ara-C cell lines in a concentration-dependent manner. The IC₅₀ value of NL-1 was comparable in REH (47.35 ± 7.7 μM) and REH/Ara-C (56.26 ± 8.8 μM) cells. Furthermore, the NL-1 treatment (60 μM; 72 hours) consistently reduced the size and number of preformed SD1 spheroids in SD1 ALL cells. Additionally, both the number of live cells and the percentage of viability of the cells was reduced when treated with NL-1. NL-1 pretreatment inhibited the chemotactic ability of both REH and REH/Ara-C cells to migrate toward multiple chemoattractants. The cells treated with NL-1 showed a dose-dependent decrease in chemotaxis in both cells. Treatment of BMSC and HOB cocultures with NL-1 (60 μM) decreased the number of live cells recovered from the S and PD cell populations and decreased viability of the PD population. Further, treatment with NL-1 (60 μM; 6 hours) increased the fluorescence intensity compared with untreated cells, indicating an increase in autophagy. In mice, the NL-1–treated group (1 mg/kg per day) showed significantly lower tumor burden than the control vehicle–treated group on day 14 postinjection^[1].
参考文献

[1] Geldenhuys WJ, Nair RR, Piktel D, Martin KH, Gibson LF. The MitoNEET Ligand NL-1 Mediates Antileukemic Activity in Drug-Resistant B-Cell Acute Lymphoblastic Leukemia. J Pharmacol Exp Ther. 2019;370(1):25-34

实验方案

计算器摩尔计算器总药量计算器工作液计算器
储存液制备
- 1 mg
- 5 mg
- 10 mg
- 1 mM
- 2.9810 mL
- 14.9049 mL
- 29.8097 mL
- 5 mM
- 0.5962 mL
- 2.9810 mL
- 5.9619 mL
- 10 mM
- 0.2981 mL
- 1.4905 mL
- 2.9810 mL

技术信息

CAS号

188532-26-5

储存条件

粉末: Sealed in dry,Store in freezer, under -20°C

液体: -20°C:3-6 月 -80°C:12月

分子式

C18H25NO3S

运输

蓝冰

分子量

335.46

别名

溶解度

DMSO	240.0 mg/mL (715.4 mM) warming

动物实验配方

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