Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.
|CP5V is a PROTAC targeting on Cdc20 with DC50 value of 1.6μM, comprising a Cdc20 ligand and VHL binding moiety bridged by a PEG5 linker. It leads to significant inhibition of breast cancer cell proliferation and resensitization of Taxol-resistant cell lines and suppressed breast tumor progression in vivo.